Plasmid

Part:BBa_K1391020:Design

Designed by: Jing Wei "Raymond" Liu   Group: iGEM14_MIT   (2014-10-17)


MAV1212_hEF1a_eBFP2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 3348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 3348
    Illegal NotI site found at 402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal BglII site found at 1694
    Illegal BglII site found at 3848
    Illegal BamHI site found at 3366
    Illegal XhoI site found at 394
    Illegal XhoI site found at 2093
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 3348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3387
    Illegal XbaI site found at 3360
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 3348
    Illegal NgoMIV site found at 645
    Illegal NgoMIV site found at 926
    Illegal NgoMIV site found at 1828
    Illegal AgeI site found at 774
    Illegal AgeI site found at 1206
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3131
    Illegal BsaI.rc site found at 3749


Design Notes

This part was created through Gateway Reaction (LR) of pENTR_hEF1a and pENTR_eBFP2 into MAV1212-RFP.

Source

hEF1a is of human origin.

References